DNA Ligation Calculator
Calculate optimal DNA ligation conditions including insert-to-vector ratios, DNA amounts, and ligase concentrations for molecular cloning.
DNA Parameters
bp
Length of vector DNA in base pairs
bp
Length of insert DNA in base pairs
Vector DNA concentration
Insert DNA concentration
Molar ratio of insert to vector
µL
Total ligation reaction volume
Ligation Results
Enter DNA parameters to calculate ligation conditions
DNA Ligation Guidelines
Optimal Conditions
- • Insert-to-vector ratio: 2:1 to 5:1
- • Total DNA: 50-200 ng per reaction
- • T4 DNA Ligase: 1-5 units
- • Incubation: 16°C overnight or RT 1-4 hours
Tips for Success
- • Use fresh, high-quality DNA
- • Ensure compatible sticky ends
- • Include appropriate controls
- • Optimize ratio based on insert size